Global Positioning System Applications

The space-based Navstar Global Positioning System (GPS), which is scheduled to achieve full operational status by late 1988, will consist of 18 active satellites placed around six orbital rings at 10,898 nautical miles above the earth. Every other ring will contain an extra satellite that will function as an active on-orbit spare. This regular and precise constellation of GPS satellites will provide continuous, three-dimensional global navigation coverage to users worldwide. Average positioning accuracies of 15 meters or less are anticipated by the military. This extraordinary precision has been demonstrated repeatedly under field-test conditions in both the United States and Western Europe. Other tests indicate that relative (differential) navigation, which employs user sets rigged to communicate their navigation solution to one another, can achieve substantially better results. Relative errors of only 1 or 2 meters have been demonstrated with existing equipment under realistic field-test conditions

Phosphorylation of CENP-A on serine 7 does not control centromere function

CENP-A is the histone H3 variant necessary to specify the location of all eukaryotic centromeres via its CENP-A targeting domain and either one of its terminal regions. In humans, several post-translational modifications occur on CENP-A, but their role in centromere function remains controversial. One of these modifications of CENP-A, phosphorylation on serine 7, has been proposed to control centromere assembly and function. Here, using gene targeting at both endogenous CENP-A alleles and gene replacement in human cells, we demonstrate that a CENP-A variant that cannot be phosphorylated at serine 7 maintains correct CENP-C recruitment, faithful chromosome segregation and long-term cell viability. Thus, we conclude that phosphorylation of CENP-A on serine 7 is dispensable to maintain correct centromere dynamics and function

Disruption of the Hippocampal and Hypothalamic Blood-Brain Barrier in a Diet-Induced Obese Model of Type II Diabetes: Prevention and Treatment by the Mitochondrial Carbonic Anhydrase Inhibitor, Topiramate

Background: Type II diabetes is a vascular risk factor for cognitive impairment and increased risk of dementia. Disruption of the blood–retinal barrier (BRB) and blood–brain barrier (BBB) are hallmarks of subsequent retinal edema and central nervous system dysfunction. However, the mechanisms by which diet or metabolic syndrome induces dysfunction are not understood. A proposed mechanism is an increase in reactive oxygen species (ROS) and oxidative stress. Inhibition of mitochondrial carbonic anhydrase (mCA) decreases ROS and oxidative stress. In this study, topiramate, a mCA inhibitor, was examined for its ability to protect the BRB and BBB in diet-induced obese type II diabetic mice. Methods: BBB and BRB permeability were assessed using 14C-sucrose and 99mTc-albumin in CD-1 mice fed a low-fat (control) or a high-fat diet. Topiramate administration was compared to saline controls in both preventative and efficacy arms examining BRB and BBB disruption. Body weight and blood glucose were measured weekly and body composition was assessed using EchoMRI. Metabolic activity was measured using a comprehensive laboratory animal monitoring system. Brain tissues collected from the mice were assessed for changes in oxidative stress and tight junction proteins. Results: High-fat feeding caused increased entry of 14C-sucrose and 99mTc-albumin into the brains of diet-induced obese type II diabetic mice. Increased permeability to 14C-sucrose was observed in the hypothalamus and hippocampus, and attenuated by topiramate treatment, while increased permeability to 99mTc-albumin occurred in the whole brain and was also attenuated by topiramate. Treatment with topiramate decreased measures of oxidative stress and increased expression of the tight junction proteins ZO-1 and claudin-12. In the retina, we observed increased entry of 99mTc-albumin simultaneously with increased entry into the whole brain during the preventative arm. This occurred prior to increased entry to the retina for 14C-sucrose which occurred during the efficacy arm. Treatment with topiramate had no effect on the retina. Conclusions: Blood–brain barrier and blood–retinal barrier dysfunction were examined in a mouse model of diet-induced obese type II diabetes. These studies demonstrate that there are spatial and temporal differences in 14C-sucrose and 99mTc-albumin permeability in the brain and retina of diet-induced obese type II diabetic mice. Topiramate, a mitochondrial carbonic anhydrase inhibitor, is efficacious at both preventing and treating BBB disruption in this diet-induced obese type II diabetic mouse model

Einstein's "Zur Elektrodynamik..." (1905) Revisited, with Some Consequences

Einstein, in his "Zur Elektrodynamik bewegter Korper", gave a physical (operational) meaning to "time" of a remote event in describing "motion" by introducing the concept of "synchronous stationary clocks located at different places". But with regard to "place" in describing motion, he assumed without analysis the concept of a system of co-ordinates. In the present paper, we propose a way of giving physical (operational) meaning to the concepts of "place" and "co-ordinate system", and show how the observer can define both the place and time of a remote event. Following Einstein, we consider another system "in uniform motion of translation relatively to the former". Without assuming "the properties of homogeneity which we attribute to space and time", we show that the definitions of space and time in the two systems are linearly related. We deduce some novel consequences of our approach regarding faster-than-light observers and particles, "one-way" and "two-way" velocities of light, symmetry, the "group property" of inertial reference frames, length contraction and time dilatation, and the "twin paradox". Finally, we point out a flaw in Einstein's argument in the "Electrodynamical Part" of his paper and show that the Lorentz force formula and Einstein's formula for transformation of field quantities are mutually consistent. We show that for faster-than-light bodies, a simple modification of Planck's formula for mass suffices. (Except for the reference to Planck's formula, we restrict ourselves to Physics of 1905.)Comment: 55 pages, 4 figures, accepted for publication in "Foundations of Physics

Multiscale Soil Investigations: Physical Concepts And Mathematical Techniques

Soil variability has often been considered to be composed of “functional” (explained) variations plus random fl uctuations or noise. However, the distinction between these two components is scale dependent because increasing the scale of observation almost always reveals structure in the noise (Burrough, 1983). Soils can be seen as the result of spatial variation operating over several scales, indicating that factors infl uencing spatial variability differ with scale. Th is observation points to variability as a key soil attribute that should be studied